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KMID : 1177720130070010035
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Journal of Alternatives to Animal Experiments
2013 Volume.7 No. 1 p.35 ~ p.38
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Basal expression of pro-inflammatory cytokines in a reconstructed human epidermis developed in Korea
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Jo Ji-Hoon
Roque Katharine
Shin So-Jung
Cho Ah-Rang
Heo Yong
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Abstract
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A reconstructed human epidermis, Keraskin™ developed in Korea, was evaluated for basal production of pro-inflammatory cytokines, interleukin (IL)-1α, IL-6, IL-8, and IL-18 in the absence of stimulators. Keraskin™ tissues in culture plate insert were incubated in 5% CO2 incubator in the presence of DMEM culture media containing 10% fetal bovine serum for 24, 48, 72, and 96 hours. Cutlure supernatants and Keraskin™ tissue lysates were obtained at the each incubation period. Level of each cytokine was determined using a sandwich ELISA method. Comparing production of IL-1α and IL-8 in culture supernatants after 24 hour incubation, level of these cytokines was significantly augmented at 48, 72, and 96 hours without statistical difference among these incubation periods. IL-18 production level in culture supernatants was the highest after 48 hour incubation. Levels of IL-1α, IL-8, and IL-18 in cell lysates were similar at the all culture periods. Level of IL-6 in both samples was below detection limit, which requires further investigation. Overall, Keraskin™ could be a valuable reconstructed human epidermis for alternative test for skin sensitization screening.
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KEYWORD
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reconstructed human epidermis, interelukin-1α, interleukin-8, interleukin-18
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